Chamber can mean several things:

• Chamber of commerce, a business network
• Chambers of parliament, in politics
• Chamber (comics), a superhero from Marvel Comics
• Chamber music, a form of classical music
• Chamber (firearms), the part of the gun's barrel in which the cartridge is placed
• A room inside a building
  • Chambers (law), the rooms used by a barrister in a court
• Combustion chamber, part of an engine in which fuel is burned