Sequence analysis in molecular biology involves identifying the sequence of nucleotides in a nucleic acid, or amino acids in a peptide or protein. Once a sample has been obtained, DNA sequences may be produced automatically by machine and the result displayed on computer. Interpreting those results is still a task for humans.

Information from sequence analysis is used in many fields of biology. It gives information on the relationship between individual organisms, or between groups of organisms. It shows how closely related they are.